Predicting transgressive segregants in early generation using single seed descent method derived micromacrosperma genepool of lentil lens culinaris medikus. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. We use discreet stealth packaging and have a very good track record of sending seeds to all our customers. Heterosis and inbreeding depression in two soybean single. The single seed descent ssd method of inbreeding minimizes the amount of genetic sampling.
Crop science abstract simulated comparisons of single. Comparative efficiency of pedigree, modified bulk and. Four populations of linseed derived from five parents were advanced from the f2 to the f6 generation by ssd and to the f5 by pedigree selection. The average c t value observed among the single seed samples was 24. Abstract breeding crosses from the germplasm enhancement of maize gem project between exotic accessions and elite corn belt dent inbreds provide a unique opportunity for broadening the genetic ba. Seed germination processes and requirements d id you know that the double coconut palm produces the largest seed in the world. Single seed descent ssd procedure for single seed descent method. There are many selection methods, but no method is perfect for general use with all crop plants. A modified pedigree method of selection in soyabeans. First proposed by goulden 1939 and later modified by brim 1966. It speeds up the breeding cycle, increases the number of favorable genotypes, and reduces breeding costs. Comparison of random bulk population and singleseeddescent. The comparison was made in hill plot tests over a 2yr period at two locations.
Singleseed descent, singlepod, and bulk sampling methods. Genetical consequences of single seed descent in the breeding of. We have developed the search option singleseedderived germplasm only to enable users to limit their search results. Ideotype breeding for submergence tolerance and cooking. The efficiency of pedigree and single seed descent.
He noted that a wheat breeding program could be divided into two stages. It involved the production and trialling in 1991 of 95 f6 families from a single cross made in 1985 between lines derived from cultivars criffel and. A comparison of the efficiency of four breeding methods, the pedigree, modified pedigree and bulk methods, and single seed descent, with a. Singleseed descent, singlepod, and bulk sampling methods for. Centres of origin, plant biodiversity and its conservation. Pedigree selection was the most effective simulated selection. Whatever the size of the seed, the function is to grow into mature plants. Typically, a single cross between two good cultivars is followed by rapid inbreeding, derivation of f 4,f 5,orf 6 lines, line evaluation, and selection. Rapid generation advance rga with single seed descent is an effective tool for rice breeders. Description two parental lines are crossed to generate f1 progeny which are then selfed to produce f2 plants. The f 2 derivedfamily method involved elimination of lowyielding f 2 derived families in replicated yield trials in f 4, f 5 and f 6. Single seed descent method this is the classic procedure of having a single seed from each plant, bulking the individual seeds, and planting out the next generation. Ps and single seed descent ssd methods in two yardlong bean. Single seed descent t aaaax aaaa 183f southern blotting dna dna dna rna rna, rna trna dna, atp dna rna.
The ssd method is an efficient costsaving method of advancing lentil populations and is recommended for lentil breeding. Ppt plant breeding methods powerpoint presentation. Breeders must carefully consider which method will be the most effective for their purposes. Single seed descent ssd is a method that enables plant breeders to advance generations while maintaining genetic variability johnson and bernard 1962. The results are presented of two single seed descent ssd breeding programmes for swedes brassica napus l. Genetics congress although he did not use the term single seed descent. Only then is line selection performed, regardless of the pedigree. Please view the pdf by using the full text pdf link under view to the left.
Singleseed descent was conducted from the f2 through f5 generation, at which time seed was bulked from a single plant per line as source material for recombinant inbred lines ril. A comparison of the efficiency of four breeding methods, the pedigree, modified pedigree and bulk methods, and single seed descent, with a pair of crosses whose potential was assessed in a previous paper, showed that, on average, the f 6 products of the three methods in which selection had been practiced outscored those produced by single seed descent for most characters for which. Breeding of vegetable tuber and spice crops icar ecourse. Repeated selfing or singleseed descent is the fastest way to achieve homozygosity with a group or family. Description two parental lines are crossed to generate f1. Field and computer simulation studies were used to estimate breeding progress with pedigree selection, single seed descent ssd and combinations of early generation pedigree selection followed by ssd. Continuity of method performance was evaluated across three spring barley populations. The performance of 52 doubled haploid dh lines from two barley crosses was compared with lines developed by the pedigree pd and the single seed descent ssd methods.
Comparison of conventional, modified single seed descent. Comparative efficiency of pedigree, modified bulk and single seed descent breeding methods of selection for developing highyielding lines in rice oryza sativa l. Pedigree selection was the most effective simulated selection method at high 75% and moderate 50% heritabilities. The theoretical changes in the mean and variance of a segregating population under single seed descent, a method of rapid generation. Instead of bulking whole seed lot of selected plants, a single seed is selected randomly from each selected plant to make bulk.
As well as over 40 free seed promotions, seedsman gives you up to 20% off your order depending on its size. Selection is postponed in the second procedure to a more homozygous, f 6 generation, which is obtained by single seed descent. The single pod descent spd and bulk methods bm produce redundant inbred lines that are descended from either the same f2 or f3 plant. Single seedbased genotyping of rice grains with different physicalchemical properties. Relationship of dough extensibility to dough strength in a. This method uses estimates of the mean m and additive genetic variance d of a. Pdf the efficiency of pedigree and single seed descent. In soybean, ssd was found to be more efficient than pedigree selection for yield improvement. The results showed that immature 35dayold seeds can be used successfully to turn over a generation of pigeonpea with 100% seed germination. Single seed descent method this is the classic procedure of having a single seed from each plant, bulking the individual seeds, and. The aim of the ssd procedure is to make many homozygous lines in a short time at a low cost. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. Genetical consequences of single seed descent in the breeding of selfpollinating crops. Winner of the standing ovation award for best powerpoint templates from presentations magazine.
The idea of this method was first suggested by goulden 1941 and subsequently modified by brim 1960. The first programme produced cultivar virtue and was done as part of a research programme on heterosis. Two plant breeding methods generally used in breeding selfpollinated crops, the bulkpopulation bp and the singleseeddescent ssd methods, compared by computer simulation to determine which retained the most additive genetic variation after four generations of inbreeding. Pdf on jan 1, 20, malinee janwan and others published rice breeding for high yield by advanced single. Through the single seed descent ssd method, a large population of recombinant inbred lines rils was developed, and we demonstrated the potential of using markerassisted selection mas in the identification of the ideotype from the offspring. Watch video lecture of principles of plant breeding icar e course free pdf book download. The singlepod descent spd and bulk methods bm produce redundant inbred lines that are descended from either the same f2 or f3 plant. The lines differed for height due to segregation at rht2. A theoretical comparison of diploidised haploid and single seed.
Principles of plant breeding icar e course free pdf book download e krishi shiksha iari toppers team june 03. Pods containing 2 to 3 seed are harvested and bulked from soybean plants in segregating populations. We are planning to advance the material by the singleseeddescent method and to accumulate accurate phenotype data for these accessions. The results showed minimum differences between pedigree and ssd lines for. Mutation method f cdna asymmetric t real time y laci s laci activator inverse pcr touchdown pcr realtime pcr northern blotting t rnai. A breeding procedure used with segregating populations of selfpollinated species in which plants are advanced by single seeds from one generation to the. Systems for making nias core collections, singleseed. October 4, 2007 at north carolina state university department of crop science. Discuss processes and requirements of seed germination. Comparison of pedigree selection and single seed descent. Single seed descent method was easier maintain the mean and higher diversity of the population compared with bulk method srivastava, 1989. Single seed descent method is the modification of bulk method of breeding. Use of immature seed germination and single seed descent.
But the modification is in such a way that it allows the equal survival of a segregates. Graduate thesis or dissertation a comparison of three. Development of uniform f1 hybrid varieties of korean. The principle of single seed descent method is an individual plant chosen from crossing at f2 and then grown only. In the bulk breeding method, some plants are selected from the f 2 population, and their seeds. To test if a single f 6 seed yielded sufficient dna to run at least ten independent snp kasp assays, c t values from 96 seed samples from 24 rice accessions were generated and analyzed. This paper reports the development of an rga technology that integrates the germination of immature seeds with single seed descent method of breeding. Comparison of mass, f2derived family, and singleseed. Definition, aim, objectives and scope of plant breeding. Pedigree method and single seed descent method authorstream. Self incompatibility and male sterility, their classification and application in crop improvement principles of breeding in selfpollinated crops, pure line selection, mass selection, heterosis breeding, hybridization. Pdf single seed descent method in plant breeding mugisha. The single seed descent method, propos ed by goulden 1939 and later m odified by grafius 1965, as a modification of the bulk breeding schem e, appears to hav e the characteristics to overcome the. Single seed descent method done by planting only one seed of one plant in one generation.
This study was conducted to compare the effectiveness between the pedigree selection ps and single seed descent ssd methods in two yardlong bean crosses. The singleseed descent method is considered a tool to exploit durum wheat genetic resources 70. Efficiency of single seed descent and early selection in the. Principles of plant breeding icar e course free pdf book. Breeding procedure of single seed descent method 3. Genetic diversity of character agronomy population single. Modes of reproduction, pollination systems and genetics of important vegetable, tuber and spice crops. The theoretical changes in the mean and variance of a segregating population under single seed descent, a method of rapid generation advancement in selfpollinating crops, are discussed. Pdf rice breeding for high yield by advanced single seed.
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